Purification:Article Title: Identification of Rab30 as a novel regulator of tubular endosomes.
Article Snippet: Chem. (2015) 290, 22250–22261 pGEX-4T-3 GE Healthcare (Chicago, IL, USA) NA934-1ML pGEX-4T-3-Rab30 RDB16853 Genes Cells (2006) 11, 1023–1037 pEF-BOS-3xFLAG-BICD2-CC3 This study pEGFP-C1-BICD2-CC3 This study pEGFP-C1-BICD2-WT This study pEGFP-C1-BICD2-WTSR This study pEGFP-C1-BICD2-DCC3 This study pEGFP-C1-BICD2-DCC3SR This study pSpCas9(BB)-2A-puro-sgRab30 Addgene, #48139: pSpCas9(BB)-2A-puro Antibodies Dilution Source Anti-MICAL-L1 mouse mAb IF (1/200) Abnova (Tipei, Taiwan) H00085377-B01P Anti-Rab30 rabbit pAb IB (1/10,000) J. .. Cell Biol. (2016) 213, 355–369 Anti-EEA1 rabbit mAb IF (1/200) Cell Signaling Technology (Danvers, MA) 3288 Anti-TfR mouse mAb IF (1/400) Thermo Fisher Scientific, 13-6800 Anti-LBPA mouse mAb IF (1/200) Echelon Biosciences (Salt Lake City, UT) Z-PLBPA Anti-Lamp1 mouse mAb IF (1/200) Santa Cruz Biotechnology (Dallas, TX) sc-20011 Anti-NogoA rabbit pAb IF (1/200) Bio-Rad, AHP1799 Anti-LC3 rabbit pAb IF (1/1,000) MBL (Nagoya, Japan) PM036 Anti-adaptinγ mouse IF (1/100) BD Transduction Lab (Franklin Lakes, NJ) 610385 Anti-GM130 mouse mAb IF (1/200) BD Transduction Lab. 610822 Anti-TGN46 rabbit pAb IF (1/400) Abcam (Cambridge, UK) ab50595 Anti-HSP90 mouse mAb IB (1/10,000) BD Transduction Lab. 610419 HRP-conjugated β-actin mouse mAb IB (1/10,000) Proteintech (Rosemont, IL) HRP-60008 Purified anti-human CD147 mouse mAb Ab uptake assay (1/100), IB (1/10,000) BioLegend (San Diego, CA), 306202 Affinipure Goat Anti-Mouse IgG (H+L) Ab uptake assay (1/20) Jackson ImmunoResearch (West Grove, PA), 115-005-003 Anti-RFP rabbit pAb IB (1/10,000) Rockland (Philadelphia, PA) 600-401-379 Anti-GFP rabbit pAb IF (1/500), IB (1/10,000) MBL, 598 Anti-BICD2 rabbit pAb IB (1/20,000) Proteintech (Rosemont, IL) 29603-1-AP Anti-Kinesin (heavy chain) mouse mAb IB (1/10,000) Sigma-Aldrich (St. Louis, MO) MAB1614 HRP-conjugated anti-mouse-IgG goat pAb IB (1/10,000) Southern Biotech (Birmingham, AL) 1031-05 HRP-conjugated anti-rabbit-IgG donkey pAb IB (1/10,000) GE Healthcare (Chicago, IL, USA) NA934-1ML Alexa Fluor 488+ anti-rabbit-IgG goat pAb IF (1/5,000) Thermo Fisher Scientific, A32731 Alexa Fluor 555+ anti-rabbit-IgG goat pAb IF (1/5,000) Thermo Fisher Scientific, A32732 Alexa Fluor 555+ anti-mouse-IgG goat pAb IF and Ab uptake assay (1/5,000) Thermo Fisher Scientific, A32727 HRP-conjugated GST tag mAb IB (1/20,000) Proteintech (Rosemont, IL) HRP-66001 Monoclonal anti-FLAG M2-HRP Ab IB (1/20,000) Sigma-Aldrich (St. Louis, MO) A8592 Ab, antibody; IB, immunoblot; IF, immunofluorescence; mAb, monoclonal Ab; pAb polyclonal Ab ..
Western Blot:Article Title: Identification of Rab30 as a novel regulator of tubular endosomes.
Article Snippet: Chem. (2015) 290, 22250–22261 pGEX-4T-3 GE Healthcare (Chicago, IL, USA) NA934-1ML pGEX-4T-3-Rab30 RDB16853 Genes Cells (2006) 11, 1023–1037 pEF-BOS-3xFLAG-BICD2-CC3 This study pEGFP-C1-BICD2-CC3 This study pEGFP-C1-BICD2-WT This study pEGFP-C1-BICD2-WTSR This study pEGFP-C1-BICD2-DCC3 This study pEGFP-C1-BICD2-DCC3SR This study pSpCas9(BB)-2A-puro-sgRab30 Addgene, #48139: pSpCas9(BB)-2A-puro Antibodies Dilution Source Anti-MICAL-L1 mouse mAb IF (1/200) Abnova (Tipei, Taiwan) H00085377-B01P Anti-Rab30 rabbit pAb IB (1/10,000) J. .. Cell Biol. (2016) 213, 355–369 Anti-EEA1 rabbit mAb IF (1/200) Cell Signaling Technology (Danvers, MA) 3288 Anti-TfR mouse mAb IF (1/400) Thermo Fisher Scientific, 13-6800 Anti-LBPA mouse mAb IF (1/200) Echelon Biosciences (Salt Lake City, UT) Z-PLBPA Anti-Lamp1 mouse mAb IF (1/200) Santa Cruz Biotechnology (Dallas, TX) sc-20011 Anti-NogoA rabbit pAb IF (1/200) Bio-Rad, AHP1799 Anti-LC3 rabbit pAb IF (1/1,000) MBL (Nagoya, Japan) PM036 Anti-adaptinγ mouse IF (1/100) BD Transduction Lab (Franklin Lakes, NJ) 610385 Anti-GM130 mouse mAb IF (1/200) BD Transduction Lab. 610822 Anti-TGN46 rabbit pAb IF (1/400) Abcam (Cambridge, UK) ab50595 Anti-HSP90 mouse mAb IB (1/10,000) BD Transduction Lab. 610419 HRP-conjugated β-actin mouse mAb IB (1/10,000) Proteintech (Rosemont, IL) HRP-60008 Purified anti-human CD147 mouse mAb Ab uptake assay (1/100), IB (1/10,000) BioLegend (San Diego, CA), 306202 Affinipure Goat Anti-Mouse IgG (H+L) Ab uptake assay (1/20) Jackson ImmunoResearch (West Grove, PA), 115-005-003 Anti-RFP rabbit pAb IB (1/10,000) Rockland (Philadelphia, PA) 600-401-379 Anti-GFP rabbit pAb IF (1/500), IB (1/10,000) MBL, 598 Anti-BICD2 rabbit pAb IB (1/20,000) Proteintech (Rosemont, IL) 29603-1-AP Anti-Kinesin (heavy chain) mouse mAb IB (1/10,000) Sigma-Aldrich (St. Louis, MO) MAB1614 HRP-conjugated anti-mouse-IgG goat pAb IB (1/10,000) Southern Biotech (Birmingham, AL) 1031-05 HRP-conjugated anti-rabbit-IgG donkey pAb IB (1/10,000) GE Healthcare (Chicago, IL, USA) NA934-1ML Alexa Fluor 488+ anti-rabbit-IgG goat pAb IF (1/5,000) Thermo Fisher Scientific, A32731 Alexa Fluor 555+ anti-rabbit-IgG goat pAb IF (1/5,000) Thermo Fisher Scientific, A32732 Alexa Fluor 555+ anti-mouse-IgG goat pAb IF and Ab uptake assay (1/5,000) Thermo Fisher Scientific, A32727 HRP-conjugated GST tag mAb IB (1/20,000) Proteintech (Rosemont, IL) HRP-66001 Monoclonal anti-FLAG M2-HRP Ab IB (1/20,000) Sigma-Aldrich (St. Louis, MO) A8592 Ab, antibody; IB, immunoblot; IF, immunofluorescence; mAb, monoclonal Ab; pAb polyclonal Ab ..
Immunofluorescence:Article Title: Identification of Rab30 as a novel regulator of tubular endosomes.
Article Snippet: Chem. (2015) 290, 22250–22261 pGEX-4T-3 GE Healthcare (Chicago, IL, USA) NA934-1ML pGEX-4T-3-Rab30 RDB16853 Genes Cells (2006) 11, 1023–1037 pEF-BOS-3xFLAG-BICD2-CC3 This study pEGFP-C1-BICD2-CC3 This study pEGFP-C1-BICD2-WT This study pEGFP-C1-BICD2-WTSR This study pEGFP-C1-BICD2-DCC3 This study pEGFP-C1-BICD2-DCC3SR This study pSpCas9(BB)-2A-puro-sgRab30 Addgene, #48139: pSpCas9(BB)-2A-puro Antibodies Dilution Source Anti-MICAL-L1 mouse mAb IF (1/200) Abnova (Tipei, Taiwan) H00085377-B01P Anti-Rab30 rabbit pAb IB (1/10,000) J. .. Cell Biol. (2016) 213, 355–369 Anti-EEA1 rabbit mAb IF (1/200) Cell Signaling Technology (Danvers, MA) 3288 Anti-TfR mouse mAb IF (1/400) Thermo Fisher Scientific, 13-6800 Anti-LBPA mouse mAb IF (1/200) Echelon Biosciences (Salt Lake City, UT) Z-PLBPA Anti-Lamp1 mouse mAb IF (1/200) Santa Cruz Biotechnology (Dallas, TX) sc-20011 Anti-NogoA rabbit pAb IF (1/200) Bio-Rad, AHP1799 Anti-LC3 rabbit pAb IF (1/1,000) MBL (Nagoya, Japan) PM036 Anti-adaptinγ mouse IF (1/100) BD Transduction Lab (Franklin Lakes, NJ) 610385 Anti-GM130 mouse mAb IF (1/200) BD Transduction Lab. 610822 Anti-TGN46 rabbit pAb IF (1/400) Abcam (Cambridge, UK) ab50595 Anti-HSP90 mouse mAb IB (1/10,000) BD Transduction Lab. 610419 HRP-conjugated β-actin mouse mAb IB (1/10,000) Proteintech (Rosemont, IL) HRP-60008 Purified anti-human CD147 mouse mAb Ab uptake assay (1/100), IB (1/10,000) BioLegend (San Diego, CA), 306202 Affinipure Goat Anti-Mouse IgG (H+L) Ab uptake assay (1/20) Jackson ImmunoResearch (West Grove, PA), 115-005-003 Anti-RFP rabbit pAb IB (1/10,000) Rockland (Philadelphia, PA) 600-401-379 Anti-GFP rabbit pAb IF (1/500), IB (1/10,000) MBL, 598 Anti-BICD2 rabbit pAb IB (1/20,000) Proteintech (Rosemont, IL) 29603-1-AP Anti-Kinesin (heavy chain) mouse mAb IB (1/10,000) Sigma-Aldrich (St. Louis, MO) MAB1614 HRP-conjugated anti-mouse-IgG goat pAb IB (1/10,000) Southern Biotech (Birmingham, AL) 1031-05 HRP-conjugated anti-rabbit-IgG donkey pAb IB (1/10,000) GE Healthcare (Chicago, IL, USA) NA934-1ML Alexa Fluor 488+ anti-rabbit-IgG goat pAb IF (1/5,000) Thermo Fisher Scientific, A32731 Alexa Fluor 555+ anti-rabbit-IgG goat pAb IF (1/5,000) Thermo Fisher Scientific, A32732 Alexa Fluor 555+ anti-mouse-IgG goat pAb IF and Ab uptake assay (1/5,000) Thermo Fisher Scientific, A32727 HRP-conjugated GST tag mAb IB (1/20,000) Proteintech (Rosemont, IL) HRP-66001 Monoclonal anti-FLAG M2-HRP Ab IB (1/20,000) Sigma-Aldrich (St. Louis, MO) A8592 Ab, antibody; IB, immunoblot; IF, immunofluorescence; mAb, monoclonal Ab; pAb polyclonal Ab ..
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